The fourth-generation Land Cruiser Prado was launched in September 2009. While the Prado had been sharing the chassis with the Hilux Surf since May 1996, the two lines finally merged as the fourth-generation Prado. In the domestic market, only long wheelbase models (2,790 mm) were made available, although short wheelbase models continued to be offered overseas. Principal mechanical components, including the suspension and the lineup of 2.7-liter 4-cylinder and 4-liter V6 engines, were unchanged from the predecessor's. New features included the crawl control system for facilitating off-road driving at a very low speed, as well as the multi-terrain select system that could optimize the drive settings for any given off-road conditions.

The second-row seats could slide back and forth to adjust the legroom, and the seat on the left automatically slid forward when the seatback was tilted forward, providing walk-in access to the third-row seats. The third-row seats could be automatically stowed or restored by a flick of a switch. The floor in front of the third-row seats was lowered by 50 mm to enhance ride comfort and utility.